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• n l, m-q A, e K, C n, t s, l A .. Light-regulated
Most relevant evolAArtínuirósCKeltAmAreroosiloBetet Al
stapled peptides to inhibit protein-protein interactions involved in clathrin-mediated
scientific endocytosis.Angew Chem Int Ed Engl. 2013 Jul 22;52(30):7704-8.

articles
• limA lm, GiAnnotti mi, redondo-morAtA l, vAle ml, mArques eF, sAnz F. Morphological
and nanomechanical behavior of supported lipid bilayers on addition of cationic sur-

factants.Langmuir. 2013 Jul 30;29(30):9352-61.

• izquierdo-serrA m, trAuner d, lloBet A, GorostizA P. Optical control of calcium-regula-
ted exocytosis.Biochim Biophys Acta. 2013 Mar;1830(3):2853-60.

• m mmP, G mi , o G , F l , A e, P J, s F , v
AdriGAliAnnottinCinsrAnCormelinuiGGAliAnzdel Alle
lJ, AlemAn C. Bioactive nanomembranes of semiconductor polythiophene and ther-
moplastic polyurethane: thermal, nanostructural and nanomechanical properties.

Polymer Chemistry. 2013;4(3):568-583.

• hoyo J, GuAus e, onCins G, torrent-BurGués J, sAnz F. Incorporation of ubiquinone in
supported lipid bilayers on ITO.J Phys Chem B. 2013 Jun 27;117(25):7498-506.

We have continued the research line on Scanning Tunneling Spectroscopy (STS)
Highlights
response of single proteins under electrochemical control. In particular, we have

measured redox fluctuations in the tunneling current of azurin that suggest limits to
the miniaturization of bioelectronic devices like single protein transistors.

In “Nanomechanics of biosystems” 2 new articles in the field have been publis-

hed: one in lipid bilayer response in the presence of surfactants (http://dx.doi.
org/10.1021/la400067n) and other in polymer scaffolds (http://dx.doi.org/10.1021/

bm4005436) in collaboration with the GBBIT-IBEC group from CIBER-BBN.

Outstanding results in the application of light-activated nanoswitches are the de-

velopment of photo switchable inhibitors of protein-protein interactions, synthetic
peptides whose structure can be regulated by light that and we term traffic light

peptides. These peptides allow controlling remotely clathrin mediated endocytosis

an important pathway for cellular uptake of nutrients and/or membrane proteins.
This research was published in Angewandte Chemie, being also featured in “facul-

ty of 1000” and attracting substantial interest in general public media (e.g. Radio,
newspapers). These results have served to apply for an ERC Proof of Concept grant

in H2020.

During 2013 the capabilities developed by Nanoxen+ project (e.g. in vivo testing

with small transparent animals) have been instrumental to demonstrate the pharma-
cological importance of a light-regulated drug, an allosteric modulator of metabotro-

pic glutamate receptors. These results are currently under review and have served

to submit an additional ERC Proof of Concept grant in H2020.
In the case of the “NanoFabry” CIBER-BBN project granted by La Marató TV3 (2011-

2013), we have refined the nanoconjugate structure, finished the test of toxicology 13
20
in the synthetic route, and began with the test in vivo murine models. In parallel, T
we have defined the best conditions for the cryogenic storage of the conjugate that OR
P
responds fine to a temperature programmed sequence. Besides, we have started RE
L
with the characterization –morphological, nanomechanical as well as the interactions A
between the nanoparticles decoration and their cargo of nanocarriers for antiapop- NU
N
totic drug transport across the Blood-Brain-Barrier, within the CIBER-BBN project A
“NanoTransBrain”.
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